Induction of lymphokine activated killer cells in serum-free medium.
نویسندگان
چکیده
منابع مشابه
Induction of lymphokine-activated killer-like cells by cancer chemotherapy
Natural cell-mediated cytotoxicity against NK-resistant target tumor cells was found in the peripheral blood of tumor-bearing patients approximately 1 mo after combined chemotherapy. The recognition specificity of these effector cells was broad and had no restriction. From the experiments of negative selection with mAbs and complements, these newly developed killer cells after chemotherapy were...
متن کاملImmunoregulation of lymphokine-activated killer cells.
The in vitro effects of Concanavalin A (Con A) and prednisolone (PRD) on the cytotoxic functions of lymphocytes and the generation of lymphokine-activated killer (LAK) cells were investigated. Con A at concentrations ranging from 1 to 40 micrograms/ml did not significantly affect the cytotoxicity of LAK cells when added directly to the effector and target cell mixture in a 4-hr 51Cr release ass...
متن کاملInduction of lymphokine-activated killer (LAK) cells against human leukemia cells.
Lymphokine-activated killer (LAK) cells are known to lyse fresh solid tumor cells in vitro [1], and clinical studies suggest that adoptive immunotherapy with LAK cells and interleukin-2 (IL-2) may be a promising approach in the treatment of solid tumors [2, 3]. As only few data are available on the LAK cell system in connection with human leukemia [4, 5], we investigated the induction of LAK ce...
متن کاملNatural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells.
Granzyme (Gzm) B-deficient mice obtained by gene targeting were used to assess the role of Gzm B in the mechanisms used by natural killer (NK) and lymphokine-activated killer (LAK) cells to destroy target cells. Gzm B-/- NK cells, LAK cells, and cytotoxic T lymphocytes (CTL) all are defective in their ability to rapidly induce DNA fragmentation/apoptosis in susceptible target cells. This defect...
متن کاملInduction of Lymphokine-activated Killer Cells in Strain 2 Guinea Pigs with Human Interleukin-21
Culture of spleen cells from strain 2 guinea pigs with Jurkat interleukin-2 (IL-2) resulted in the induction of lymphokine-activated killer (LAK) cells. Maximum LAK activity was induced using 5000 pmol of IL-2. Incubation of spleen cells with IL-2 for as little as 8 h resulted in detectable LAK activity. LAK cell activity was transient and could be restimulated by adding back IL-2. LAK cell act...
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ژورنال
عنوان ژورنال: Japanese Journal of Oral & Maxillofacial Surgery
سال: 1991
ISSN: 2186-1579,0021-5163
DOI: 10.5794/jjoms.37.779